No significant tubulin asters are present throughout the oocyte immediately following acute PLK-1 AID. Live imaging of tubulin density in an auxin-treated oocyte; corresponds to Fig. 4 B, top row. Pseudocolored video shows mean gray values of the GFP channel (GFP::tubulin and GFP::PLK-1 signals). Oocytes were dissected into meiosis media containing auxin and filmed. Microtubule asters were not observed immediately after acute AID since these oocytes were not pre-treated with auxin (n = 7, same as Video 4 but zoomed out to view the entire oocyte and pseudocolored). Images were acquired every 30 s, and time elapsed is shown in min:sec. Scale bar = 5 µm.
