Video 6.

Overall morphology of receptor-mediated mitophagy in the early phase, as revealed by electron tomography (related to Fig. 3, A–D). DRP1 KO HeLa cells were treated with DFP for 16 h and fixed with a mixture of aldehyde and osmium solutions for electron tomography. Serial EM images (115 sections) corresponding to Fig. 3 A are shown first, followed by color-segmented 3D reconstructed image (corresponding to Fig. 3, B–D) featuring a mitochondrion (pink), ER (blue), linear structures (cyan), an IM (green), and Golgi complex (pale yellow).


The mitophagy receptors BNIP3 and NIX mediate tight attachment and expansion of the isolation membrane to mitochondria

Shun-ichi Yamashita, Ritsuko Arai, Hiroshi Hada, Benjamin Scott Padman, Michael Lazarou, David C. Chan, Tomotake Kanki, and Satoshi Waguri

DOI: 10.1083/jcb.202408166
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