Dynamic MT is blocked at the plus end upon flow in of CEP97^CP110-GFP. Time-lapse movie acquired with a TIRF microscope showing in vitro–reconstituted MTs growing from GMPCPP-stabilized seeds (blue) in the presence of 15 µM tubulin and 20 nM mCherry-EB3 (gray). MT plus end is on the right side of the movie. After 5 min, CEP97^CP110-GFP (green) is flowed into the chamber along with 15 µM tubulin and 20 nM mCherry-EB3 (gray) and blocks plus-end growth. The movie is displayed at 20 frames per second. Sequential triple-color acquisition was done at an interval of 3 s over the course of 10 min. Scale bar, 2 µm.
