Lamellipodia formation during mouse neutrophils spreading. Time-lapse TIRF recording of CellMask-labelled neutrophils purified from bone marrow of a Cebpa+/+;Stim1fl/fl;Stim2fl/fl (WT) mouse. Cells were plated on PLL and observed at 100× magnification. Frames collected at 30 s intervals displayed at 4 frames/second. Related to Fig. 4 C.
