Actin dynamics during mouse neutrophils spreading. Time-lapse DIC and SiR-actin recording of bone marrow neutrophils from a Cebpa+/+;Stim1fl/fl;Stim2fl/fl (WT) mouse. Cells were labelled with Sir-Actin, plated on PLL, and observed at 63× magnification. The red fluorescence of SiR-actin increases in intensity as actin monomers assemble into microfilaments. Frames collected at 2 s intervals displayed at 8 frames/second. Related to Fig. 7 B.
