Representative live centrosome recentering event showing the repositioning of the centrosome (gray) and the centroid (magenta) inside a PtK2 cytoplast expressing GFP tubulin (Nocodazole washout, Y27632 washout, and Cytochalasin D washout) (Fig. 4). Microtubules appear in green. Nine selected time points are shown from a 4 h acquisition composed of 4-µm-wide Z-stacks (five steps spaced by 1 µm) acquired every 5 min using a confocal spinning disk microscope. The volume of the cytoplast is projected onto a single plane for all the time points. Images are processed following the described pipeline in the dedicated Materials and methods section. Playback speed: 3 frames per second.
