Repeated laser ablation experiment inside a PtK2 cytoplast expressing GFP tubulin treated with Jasplakinolide (600 nm) and Y27632 (20 µM) for 4 h (Fig. 1). Microtubules appear in green. Microtubules are extensively and repeatedly depleted on one side of the centrosome during 5 min and the motion of the centrosome is recorded in parallel. A 2-µm wide Z-stack (three steps spaced by 1 µm) is acquired every 10 s using a confocal spinning disk microscope and is then MAX projected onto a single plane. Playback speed: 5 frames per second.
