Repeated laser ablation experiment inside a PtK2 cytoplast expressing GFP tubulin (Fig. 1). Microtubules (in green) are extensively and repeatedly depleted on one side of the centrosome for 5 min and the motion of the centrosome is recorded in parallel using a confocal spinning disk microscope. Actin appears in magenta and is stained using SiR Actin. A 2-µm wide Z-stack (three steps spaced by 1 µm) is acquired every 10 s and is then MAX projected onto a single plane. Playback speed: 5 frames per second.
