Laser ablation experiment inside PtK2 cells expressing GFP tubulin (Fig. S1). Microtubules appear in green. A few microtubules are ablated on one side of the centrosome and the motion of the centrosome is recorded using a confocal spinning disk microscope during the 2 min that follow ablation. A 2 µm wide Z-stack (spaced by 1 µm) is acquired every 10 s and is then MAX projected onto a single plane. Playback speed: 4 frames per second.
