Nuclei attempt to pack tightly into apical layers after double disruption of deformation and dispersion pathways. Live imaging of kuk MT- embryos expressing RFP:NLS (false colored green) and Spider: GFP (magenta). Each frame is acquired at every 15 s and video is played at 20 frames per second. Nuclei attempt to pack together in a common apical layer but lack of deformation inhibits packing and the uniformity of cell dimensions (note small cells lacking nuclei in imaged plane). Scale bar = 10 μm.
