Nuclear crowding after double disruption of deformation and dispersion pathways. 3D segmentation generated from kuk MT- embryos at early GBE stage expressing marker for nucleus (RFP:NLS) and plasma membrane (Spider:GFP). The video displays two rotations of segmented cells and nuclei as shown in Fig. 8 A and highlights non-deformable nuclei crowding the apical layer. 30 frames/second. Scale bar = 5 μm.
