Absence of nuclear dispersion in apical–basal dimensions after perturbation of MT function. Nuclei display a “pavement-stone-like” phenotype and are in a common apical plane after MT disruption. Live imaging of colchicine-injected embryos expressing RFP:NLS (false-colored green) and Spider:GFP (magenta). Each frame is acquired every 15 s and the video is played at 20 frames per second. Scale bar = 10 μm.