Nuclei deform over the course of GBE to accommodate cell shape changes. The movie shows control nuclei labeled by the ratio of their major and minor axis lengths, as seen in Fig. 2 A. More rounded nuclei are in blue, while elongated/deformed nuclei appear in hotter colors (orange or red). Movies were acquired at a rate of 15 s per frame and displayed at 12 frames per second. Scale bar = 10 μm.