Representative time-lapse video of FRAP experiments with eGFP-COL4A2 in developing hair follicles under MMP inhibition. EGFP-COL4A2 signals (green) were photobleached in designated regions and signal recovery was monitored under MMP inhibition with batimastat. Cultured skin explants from E12.5 embryos of eGFP-Col4a2 mice were used, corresponding to Fig. 4 B, under MMP-inhibitor conditions. Live imaging was performed under a TCS SP8X (Leica) confocal microscope. Frames were collected approximately every 16 min and 13 s during the 18-h and 54-min time-lapse. The video is 1 min and 14 s in total, with the first and last frames each held for 2 s to indicate regions of interest. The remaining frames are displayed at one frame per second.
