Representative time-lapse video of pulse-chase experiments in the developing hair follicles of skin explants from mKikGR-Col4a2 mouse embryos. The green mKikGR-COL4A2 signals were photoconverted to red (magenta), and the changes in these mKikGR-COL4A2 signals were chased over ∼19 h at 30 min intervals, corresponding to Fig. 3 D. Live imaging was performed under a TCS SP8X (Leica) confocal microscope. Frames were collected approximately every 28 min and 27 s during the 18-h and 57-min time-lapse. The video is 41 s in total and displayed at one frame per second.
