Representative time-lapse video of FRAP experiments with eGFP-COL4A2 in developing hair follicles. eGFP-COL4A2 signals (green) were photobleached in designated regions and signal recovery was monitored. Cultured skin explants from E12.5 embryos of eGFP-Col4a2 mice were used, corresponding to Fig. 3 A. Live imaging was performed under a TCS SP8X (Leica) confocal microscope. Frames were collected approximately every 10 min and 7 s over the 5-h and 43-min time-lapse. The video is 37 s in total, with the first and last frames each held for 2 s to indicate regions of interest. The remaining frames are displayed at ∼0.95 frames per second.
