Video 3.

Effect of Rac2^E62K on macrophage motility in the TME. Larvae expressing mpeg1:mCherry-2A-rac2^WT or mpeg1:mCherry-2A-rac2^E62K were injected with ZMel1 cells at 2 dpf and time-lapse imaging was performed at 48 hpi. Images were acquired every 3 min for 2 h. Macrophages that interact with the tumor mass were tracked and color-coded by instantaneous speed of macrophages. Videos were generated on Imaris 10.0 and processed on premier pro. Rac2^WT (n = 5 larvae), Rac2^E62K (n = 7 larvae) from three independent replicates. Frame rate: 5 fps. Scale bar = 10 µm.

Real-time imaging reveals a role for macrophage protrusive motility in melanoma invasion

Gayathri Ramakrishnan, Veronika Miskolci, Miranda Hunter, Morgan A. Giese, Daniela Münch, Yiran Hou, Kevin W. Eliceiri, Michael R. Lasarev, Richard M. White, and Anna Huttenlocher

DOI: 10.1083/jcb.202403096