Representative movie of control (Ctr), CerS4epi−/−, and CerS4epi−/− cells transfected with CerS4-HALO imaged with Fluo-4 AM Ca2+ probe to identify oscillations. 5 μM KN93 was added at 10 min. Note that enhanced oscillations were upstream of CaMKII signaling restored to control levels by rescuing CerS4 expression. Images were aquired at 30 frames/second. Scale bars 10 μm.