Video 3.

Tomogram and segmentation of a cultured mouse neuron synapse. The video begins with a complete tomogram of the presynaptic terminal in a cultured mouse neuron. It then displays the segmentation of synaptic vesicles (SVs) using CryoVesNet, where SVs are shown in orange and connectors in black. Additional overlays illustrate the segmentation of microtubules (dark magenta), the plasma membrane (light blue), mitochondria (dark blue), and endosomes (green), providing a comprehensive annotation of the neuronal structures. 30 frames per second.


CryoVesNet: A dedicated framework for synaptic vesicle segmentation in cryo-electron tomograms

Amin Khosrozadeh, Raphaela Seeger, Guillaume Witz, Julika Radecke, Jakob B. Sørensen, and Benoît Zuber

DOI: 10.1083/jcb.202402169
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