Demonstration of Napari interactive tool usage. This video illustrates the use of our Napari-based interactive tool. The video highlights key features of the tool, including Compute Labels to segment synaptic vesicle and Remove Labels for eliminating incorrect annotations. Users can iterate through this process, refining the segmentation as needed, and correct mistakes. Once satisfied with the results, users can save the final segmentation in MRC format. Additional features include adding spherical labels for the manual addition of vesicle labels and undoing a function to revert the last modifications. 30 frames per second.