Dynamic structure of CUPS revealed by SCLIM. Cells expressing Grh1-2xmCherry and GFP-Snc2 were incubated in 2% potassium acetate for 1 h (early starvation condition) and visualized by SCLIM. The movie shows potential fusion of the Grh1-positive structure, with 10-s intervals, played at 20× speed. Grid = 0.53 μm.
