Live image of GFP-SNAP29 and mCherry-Migfilin in KP4 cells. KP4 cells were coexpressed with GFP-SNAP29 and mCherry-Migfilin. Upon starvation with EBSS for 1 h at 37°C, live-cell images were captured immediately at 37°C by Multi-SIM. GFP-SNAP29 (green) and mCherry-Migfilin (magenta) were shown, respectively. Images were collected at 0.5 frames/s and 24 frames were captured for one channel. Scale bar, 1 µm.