Liquid flows driven by ciliary beat in Kif6+/+ and Kif6−/− ependymal tissues. Related to Fig. 6 I. Fresh ependymal tissues from a pair of P36 littermates were incubated with SiR-tubulin to label cilia and fluorescent latex beads as flow indicators as illustrated in Fig. 6 H. Motilities of the beads beneath the tissues were imaged with a spinning disk confocal microscope at 100-ms intervals and at a fixed z-plane. The playback speed is 5 fps. Colored lines in the first 500 ms indicate trajectories of representative, rapidly moving fluorescent puncta.
