Light induction triggers the formation of NBs with FITmSS271AA but with different dynamics than with FIT (supports Figs. 1 and 2). Time series showing representative localization of FITmSS271AA-GFP from 0 to 20 min after the blue light trigger in the nucleus. Pictures were taken in 15-sec intervals. FITmSS271AA-GFP accumulated late in NBs (starting at 10 min), which were also smaller in size, indicating that Ser271/272 is important for proper NB formation (compare with Videos 1 and 2). Scale bar: 2 µm. G = GFP. Fluorescence protein analysis was conducted in transiently transformed N. benthamiana leaf epidermis cells, following the standardized FIT NB analysis procedure. Two independent time series experiments were conducted per construct.
