Light induction does not trigger the formation of NBs with ZAT12-GFP (supports Fig. 1). Time series showing representative localization of ZAT12-GFP from 0 to 20 min after the blue light trigger in the nucleus. Pictures were taken in 15-s intervals. ZAT12-GFP did not show NB formation and was a negative control to show that GFP did not cause the formation of NBs (compare with Videos 1 and 3). Scale bar: 2 µm. G = GFP. Fluorescence protein analysis was conducted in transiently transformed N. benthamiana leaf epidermis cells, following the standardized FIT NB analysis procedure. Two independent time series experiments were conducted per construct.
