GPI-GFP is more likely to be localized between actin rings in progenitor-derived neuronal cells. GPI-GFP was tracked (5,000 frames, 200 Hz frame rate) in progenitor-derived neuronal cells. Cells were subsequently fixed and stained for actin using phalloidin. Shown is an overlay of a STORM reconstruction of actin (magenta) and a movie of accumulated GPI-GFP localizations during 1 s (cyan) of area shown in Fig. 5 b. Scale bar is 500 nm.