Cell co-expressing mRuby2-AP-1 (red) and SBP-GFP-LAMP1 (green), treated with biotin for 28 min. AP-1 puncta can be seen on LAMP1-positive tubules, indicating that it binds to these tubules whether or not the cells are overexpressing an AP-1 cargo protein like NAGPA. The arrowheads show such both Golgi-associated and free-moving tubules. Playback speed, 9x.
