PI(4)P localization changes throughout the cell cycle. 4D confocal microscopy of cells in the indicated genetic backgrounds expressing GFP-PHOsh2. Each cell comes from a unique movie and is tiled to aid visual comparison. Movies are a single slice from the center of a Z-stack. Time is indicated in hour:min format. Still images from this video are depicted in Fig. 9 E. Scale bar, 2 µm. The frame rate is 10 fps. In the labels, the lowercase “d” is used to represent the typical ∆ symbol to indicate gene deletions. The num1∆ movie stops at 2 h and 30 min.
