Video 3.

Loss of the Num1–Scs2 interaction reduces the rate of mitochondrial division. 4D confocal microscopy video of cells expressing MitoRed (shown in grayscale) in the indicated genetic backgrounds. Fluorescence channels are merged with a bright field image. Each cell comes from a unique movie and are tiled to aid visual comparison. The video is a max projection of full Z-stacks. Time is indicated in min:s format. Still images from this video are depicted in Fig. 4 A. Scale bar, 2 µm. The frame rate is 10 fps. In the labels, the lowercase “d” is used to represent the typical ∆ symbol to indicate gene deletions.


Mitochondria–ER–PM contacts regulate mitochondrial division and PI(4)P distribution

Jason C. Casler, Clare S. Harper, Antoineen J. White, Heidi L. Anderson, and Laura L. Lackner

DOI: 10.1083/jcb.202308144
Download Original Video
powered by Glencoe Software