Num1∆FFAT tethers mitochondrial but not ER membranes over time. 4D confocal microscopy video of cells expressing Num1∆FFAT-Halo, GFP-HDEL, and MitoRed in an lnp1∆ background. The video is a max of a full Z-stack. The individual Num1∆FFAT-Halo channel is shown in grayscale beneath the merged video. Time is indicated in min:s format. Still images from this video are depicted in Fig. 3 F. Scale bar, 2 µm. The frame rate is 10 fps. Num1∆FFAT-Halo is annotated as “Num1dFFAT-Halo” in the video.
