Num1 tethers mitochondrial and ER membranes over time. 4D confocal microscopy video of cells expressing Num1-Halo, GFP-HDEL, and MitoRed in an lnp1∆ background. The video is a max projection of a full Z-stack. The individual Num1-Halo channel is shown in grayscale beneath the merged video. Time is indicated in min:s format. Still images from this video are depicted in Fig. 3 E. Scale bar, 2 µm. The frame rate is 10 fps.
