3D invasion of a singles spheroid treated with CM from Lama3 KO leader clone C1. Live-cell imaging of a 4T1 singles spheroid (1,000 cells) treated with CM from Lama3 KO leader clone C1. Spheroids are embedded in 3.0 mg/ml rat-tail collagen type I. Images were acquired every 20 min. Time scale in seconds. Movie displays 3 frames per second. End-point still of the movie is found in Fig. 4 C.
