Beating second chamber of the cardiac tube (heart) of adult Drosophila (dot-Gal4; tub-Gal80ts) reared on HSD. Video was captured under bright-field using a QImaging optiMOS scMOS camera R696 M-16-C with a 20× water immersion lens (Carl Zeiss). The neighboring PCs and fat tissue were removed during sample preparation to clearly access the cardiac tube for video recording. The video was captured at 100 fps for a duration of 10 s. Playback speed is 100 fps. This video is related to Fig. 2 J.