(Associated with Fig. 5 D). IQGAP1 promotes the dynamic turnover of actin filament end-binding proteins. Reactions contain: actin filament seeds (10% Dylight 405 label stabilized with 132 nM Alexa 405 phalloidin), 10 nM 549-SNAP-mDia1(ΔDAD), 10 nM 649-SNAP-Capping Protein (CP) with or without 488-SNAP-IQGAP1. Arrows highlight free actin filament plus ends (purple) and the presence of molecules including IQGAP1 (green), mDia1(∆DAD) (pink), or CP (yellow), formin-CP decision complexes (orange), or DC with IQGAP1 (turquoise). DC, formin-CP decision complexes. Playback, 10 FPS. Scale, 2 μm.
