(Associated with Fig. 3 E). SNAP-IQGAP1(BAD) binds the sides of actin filaments and does not interfere with filament elongation. Reactions contain 1 μM actin monomers (10% Alexa 647 label) and 75 nM 488-SNAP-IQGAP1, 488-SNAP-IQGAP1(BAD), or 488-SNAP-IQGAP1(160-end). Panels show individual wavelengths and merge. Arrows indicate the absence (purple) or presence of IQGAP1 proteins (green) on filament ends or sides. Playback, 10 FPS. Scale, 2 μm.
