(Associated with Fig. 1 D). Example of IQGAP1-mediated pause to actin filament elongation. Reactions contain 1 μM actin monomers (20% OG labeled) with or without 75 nM IQGAP1. Arrows depict active filament growth (green) or pauses to elongation (pink). Playback, 10 FPS. Scale, 2 μm.
