JL treatment with NM2 appearance. Sum intensity projection of a z-stack time-lapse of EGFP-NM2A knock-in cell expressing 3x-mScarlet-FTractin. Lamellar protrusion imaged with a 5-s frame rate. The leading edge is in the top right corner with retrograde flow to the bottom left. Actin is in gray LUT and NM2A in purple LUT. Drug treatment indicated in orange text at the first frame of treatment. Frame rate = 20 fps. Scale bar = 5 μm. Time = mm:ss.
