NM2 monomer recruitment jumpstarts NM2 filament assembly. Sum intensity projection of a z-stack time-lapse of Halo-NM2A knock-in cell expressing GFP-Stargazin-Lov-SsrA and SspB-mApple-NM2A. Lamellar protrusion imaged with a 10-s frame rate. The leading edge is in the top right corner with retrograde flow to the bottom left. Recruitable (SspB-mApple) NM2 is shown in the left panel, endogenous (Halo-NM2) in the middle panel, and an overlay with recruitable in blue and endogenous in purple in the right panel. The orange circle marks the activation ROI that was stimulated after each acquisition frame. Frame rate = 50 fps. Scale bar = 5 μm. Time = mm:ss.
