Imaging of ER-mitochondrial dynamics in U2OS cells with rapamycin-induced REEP5-MFN2 irreversible binding, corresponding to Fig. S2 H. Time-lapse images showing over 12 min of a live U2OS cell transfected with FRB-mEmerald-REEP5, FKBP-HaloTag-MFN2 (red), and mCherry-KDEL (green). 20 nM rapamycin was added at the indicated time point. Frames were captured every 19.2 s using a laser-scanning confocal microscope. The video is shown at 80 frames/s. Bars: 2 µm.
