Microtubule depolymerization and regrowth at centrosomes in control cells. Video showing the Jupiter-mCherry signal (marking microtubules) within a living control cell during a cooling–warming experiment. Cooling to 5°C begins at −174 s and warming to 20°C begins at 0 s. Note how the Jupiter-mCherry signal decreases gradually during cooling and then recovers immediately at the two centrosomes during warming.