Representative live-cell foci imaging movies of EBSS-treated U2OS cells expressing 3xFLAG-HaloTagged WIPI2 labeled with JF646 and GFP-P62. Right panel is a merge of Halo-WIPI2 (magenta) and GFP-P62 (green). Images were acquired at 20 frames per minute. 300 × 300 pixels with a pixel size of 0.27 μm.
