Activation of dynein upon dextran vesicle binding. HeLa cell expressing mDHC-GFP (green), after uptake of A647-tagged 10 kD dextran (magenta) imaged using HILO microscopy. The white arrowhead points to a dextran vesicle onto which a dynein molecule bound (indicated by an abrupt appearance of intensity in the GFP channel) followed by movement of the dynein–cargo complex toward the minus end of MTs. “N” marks the position/direction of the nucleus. Imaged at 10 fps, movie playback 10 fps. Scale bar: 5 µm. Movie related to Fig. 6, a and b.
