Dynamics of p150. HeLa cell expressing GFP-p150 imaged using HILO microscopy. Unlike dynein, whose association with the MT is short lived, p150 punctae are visible for longer durations. The green arrowhead points to a p150 puncta moving toward the minus end of MT, presumably as part of the tripartite complex. The magenta arrowhead points to a p150 puncta moving away from the nucleus, likely at the MT plus end, and a stationary p150 spot is indicated by the white arrowhead. “N” marks the position/direction of the nucleus. Imaged at 50 fps, movie playback 50 fps. Scale bar: 5 µm. Movie related to Fig. 3, f–h.
