Video 1.

Macropinocytosis in HEK293 cells expressing GFP-SnxA (white) directly following addition of 2.5 mg/ml Texas-Red dextran (red), related to Fig. 3 G. Images were captured every 60 s by spinning-disc microscopy and displayed at four frames per second. Scale bar = 2 μm.


A PI(3,5)P2 reporter reveals PIKfyve activity and dynamics on macropinosomes and phagosomes

James H. Vines, Hannes Maib, Catherine M. Buckley, Aurelie Gueho, Zhou Zhu, Thierry Soldati, David H. Murray, and Jason S. King

DOI: 10.1083/jcb.202209077
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