FIB-SEM revealed a spherical compartment of vesicles in ATG2 DKO cells. FIB-SEM from Fig. 1 moving down the Z axis of the cluster of vesicles in ATG2 DKO HEK293 cells. Each frame is a FIB-SEM slice. Scale bar: 500 nm. FIB-SEM slice thickness: 7 nm.