Video 1.

FIB-SEM revealed a spherical compartment of vesicles in ATG2 DKO cells. FIB-SEM from Fig. 1 moving down the Z axis of the cluster of vesicles in ATG2 DKO HEK293 cells. Each frame is a FIB-SEM slice. Scale bar: 500 nm. FIB-SEM slice thickness: 7 nm.


ATG9 vesicles comprise the seed membrane of mammalian autophagosomes

Taryn J. Olivas, Yumei Wu, Shenliang Yu, Lin Luan, Peter Choi, Emily D. Guinn, Shanta Nag, Pietro V. De Camilli, Kallol Gupta, and Thomas J. Melia

DOI: 10.1083/jcb.202208088
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