Video 1.

Ground truth annotations for mitochondria, ER, and Golgi apparatus. Passing through a FIB-SEM volume with contrast equalized using CLAHE. Image is from Cell 1 HEK293A prepared by CF and imaged at ∼5 nm isotropic resolution. The video shows ground truth annotations for mitochondria (cyan), ER (red), and Golgi apparatus (green). The annotations were generated for all mitochondria and Golgi apparatus within the FIB-SEM volume, and all ER within the highlighted 8 × 3 × 3 µm block (orange box).

Deep neural network automated segmentation of cellular structures in volume electron microscopy

Benjamin Gallusser, Giorgio Maltese, Giuseppe Di Caprio, Tegy John Vadakkan, Anwesha Sanyal, Elliott Somerville, Mihir Sahasrabudhe, Justin O’Connor, Martin Weigert, and Tom Kirchhausen

DOI: 10.1083/jcb.202208005