Time-lapse confocal microscopy showing of interactions between SGs and lysosomes during lysosomal damage. U2OS cells expressing mCherry-G3BP1 and GFP-LAMP1 were incubated with 2 mM LLOMe during live-cell fluorescence imaging. Red color represents mCherry-G3BP1; green color represents GFP-LAMP1. Image stacks acquired every 30 s. Video plays 6 frames/s; Time is shown in seconds. Related to Fig. S2 E.
