Localization of ARHGAP17 and Cdc42 activity at an individual invadopodia. Live TIRF imaging of an individual invadopodia from a SUM159 cells expressing TKS5-mTagBFP2, ARHGAP17-GFP (expressed in ARHGAP17 KO background), and dTomato-wGBD, treated with PDBu (scale bar, 1 μm). Frames were collected every 30 s (frame rate: 4 frames/second). Associated with Fig. 7 B.