dTomato does not localize to invadopodia. Live TIRF imaging of a SUM159 cell expressing TKS5-mTagBFP2 and dTomato, treated with PDBu (scale bar, 10 μm). Frames were collected every 30 s for 30 min with PDBu treatment at frame 5 (frame rate: 12 frames/second). Associated with Fig. S6 J.
