Live-cell microscopy of prolonged mitochondria–lysosome tethering by Mid51. Confocal time-lapse microscopy of prolonged mitochondria–lysosome tethering in Mid51 (WT) condition in a live HeLa cell expressing Lamp1-mGFP (lysosome; green) and mCherry-Mid51 (mitochondria; red). Video was acquired at 1 frame/2 s for 108 s and played back at 12 frames/s (24× speed). Video corresponds to Fig. 5 D. Scale bar, 0.5 µm.
